Das übergeordnete Ziel von FG1.2 ist die Identifizierung und funktionelle Charakterisierung der Gen- und Protein-Funktionen, die die Pflanzenproduktivität unter Stress- und Nicht-Stress-Bedingungen bestimmen. Dazu gehört auch die Charakterisierung molekularer und metabolischer Komponenten der Stressverarbeitung und Akklimatisierung. Um dieses Ziel zu erreichen, wird eine Kombination aus molekularen und zellbiologischen Ansätzen in Modellsystemen (zum Beispiel Arabidopsis thaliana) und Kulturpflanzen genutzt. Dieser Ansatz ermöglicht ein grundlegendes Verständnis regulatorischer Mechanismen, die durch umweltbedingte Anpassungen ausgelöst werden. Innerhalb der nächsten Jahre werden zwei Hauptobjekte im Vordergrund stehen: (1) Identifizierung und Charakterisierung von neuen Komponenten der pflanzlichen Antwort auf Stress, zum Beispiel im Rahmen des „SnRK1/low-energy signalling“, und (2) Nutzung von mikrobiellen Effektorproteinen, um pflanzliche zelluläre und metabolische Funktionen zu untersuchen. Ein mittelfristiges Ziel ist, diese beiden Forschungsansätze in einem Projekt zusammenzuführen, das die Kreuzregulation des Wachstums und der Abwehr untersucht. Langfristig wird ein verbessertes Verständnis der Stresstoleranz bei Pflanzen helfen, widerstandsfähigere Kulturen und nachhaltigere Anbaumethoden zu entwickeln.
1.2 Pflanzenmetabolismus
1.2 Pflanzenmetabolismus Um sinnvolle Strategien für eine verbesserte Stresstoleranz von Kulturen zu entwickeln, ist ein Verständnis der zellulären und molekularen Prozesse in der Pflanze unter biotischem und abiotischem Stress notwendig.
Dr. rer. nat.
Suayib Üstün
Großbeeren
033701 / 78 - 235
uestuen@igzev.de
Wissenschaftler*in
KOOPERATIONSPARTNER
STOREKEEPER RELATED 1/G-element Binding Protein (STKR1) interacts with protein kinase SnRK1
Nietzsche, M.; Guerra, T., Alseekh, S.; Wiermer, M.; Sonnewald, S.; Fernie, A.R.; Börnke, F. 2018. STOREKEEPER RELATED 1/G-element Binding Protein (STKR1) interacts with protein kinase SnRK1. Plant Physiology.
DOI: 10.1104/pp.17.01461
→Thigmomorphogenesis – Control of plant growth by mechanical stimulation
Börnke, F.; Rocksch, T. 2018. Thigmomorphogenesis – Control of plant growth by mechanical stimulation. Scientia Horticulturae 234, 344-353.
DOI: 10.1016/j.scienta.2018.02.059
→A proteomic approach suggests unbalanced proteasome functioning induced by the growth-promoting bacterium Kosakonia radicincitans in Arabidopsis.
Witzel, K.; Üstün, S.; Schreiner, M.; Grosch, R.; Börnke, F.; Ruppel, S. 2017. A proteomic approach suggests unbalanced proteasome functioning induced by the growth-promoting bacterium Kosakonia radicincitans in Arabidopsis. Frontiers in Plant Science 2017 8:661.
→Ubiquitin Proteasome Activity Measurement in Total Plant Extracts.
Üstün, S.; Börnke, F. 2017. Ubiquitin Proteasome Activity Measurement in Total Plant Extracts. Bio-protocol 7(17): e2532.
DOI: 10.21769/BioProtoc.2532.
→A Two-Faced Cochaperone Involved in Pattern Recognition Receptor Maturation and Viral Infection
Lamm, C.; Kraner, M.; Hofmann, J.; Börnke, F.; Mock, H.P.; Sonnewald U. 2017. Hop/Sti1 – A Two-Faced Cochaperone Involved in Pattern Recognition Receptor Maturation and Viral Infection. Front Plant Sci. 2017 Oct 11;8:1754.
DOI: 10.3389/fpls.2017.01754. eCollection 2017.
→A protein-protein interaction network linking the energy-sensor kinase SnRK1 to multiple signaling pathways in Arabidopsis thaliana.
Nietzsche, M.; Landgraf, R.; Tohge, T.; Börnke, F. 2016. A protein-protein interaction network linking the energy-sensor kinase SnRK1 to multiple signaling pathways in Arabidopsis thaliana. Current Plant Biology, 5, 36-44.
doi: 10.1016/j.cpb.2015.10.004.
http://www.sciencedirect.com/science/article/pii/S2214662815000158
→Quantitative phosphoproteomics reveals the role of the AMPK plant ortholog SnRK1 as a metabolic master regulator under energy deprivation.
Nukarinen E.; Nägele T.; Pedrotti L.; Wurzinger B.; Mair A.; Landgraf R.; Börnke F.; Hanson J.; Teige M.; Baena-Gonzalez E.; Dröge-Laser W.; Weckwerth W. 2016. Quantitative phosphoproteomics reveals the role of the AMPK plant ortholog SnRK1 as a metabolic master regulator under energy deprivation. Scientific Reports 6: 31697.
doi:10.1038/srep31697
→The proteasome acts as a hub for plant immunity and is targeted by Pseudomonas type-III effectors.
Üstün, S.; Sheikh, A.; Gimenez-Ibanez, S.; Jones, A.; Ntoukakis, V.; Börnke, F. 2016. The proteasome acts as a hub for plant immunity and is targeted by Pseudomonas type-III effectors. Plant Physiology 2016, 172 (2).
http://www.plantphysiol.org/content/early/2016/09/09/pp.16.00808.abstract
doi:10.1104/pp.16.00808
→The Xanthomonas campestris type III effector XopJ proteolytically degrades proteasome subunit RPT6.
Üstün, S.; Börnke, F. 2015. The Xanthomonas campestris type III effector XopJ proteolytically degrades proteasome subunit RPT6. Plant Physiology 116, 107-119.
→The Xanthomonas effector XopJ triggers a conditional hypersensitive response upon treatment of N. benthamiana leaves with salicylic acid.
Üstün, S.; Bartetzko, V.; Börnke, F. 2015. The Xanthomonas effector XopJ triggers a conditional hypersensitive response upon treatment of N. benthamiana leaves with salicylic acid. Frontiers in Plant Science, DOI:10.3389/fpls.2015.00599.
http://journal.frontiersin.org/article/10.3389/fpls.2015.00599/abstract
Loss of the two major leaf isoforms of sucrose-phosphate synthase in Arabidopsis thaliana limits sucrose synthesis and nocturnal starch degradation but does not alter carbon partitioning during photosynthesis.
Volkert, K.; Debast, S.; Voll, L.M.; Voll, H.; Schießl, I.; Hofmann, J.; Schneider, S.; Börnke, F. 2014. Loss of the two major leaf isoforms of sucrose-phosphate synthase in Arabidopsis thaliana limits sucrose synthesis and nocturnal starch degradation but does not alter carbon partitioning during photosynthesis. Journal of Experimental Botany 65 (18), 5217-5229.
→
Redox-activity of thioredoxin z and fructokinase-like protein 1 is dispensable for autotrophic growth of Arabidopsis thaliana.
Wimmelbacher, M.; Börnke, F. 2014. Redox-activity of thioredoxin z and fructokinase-like protein 1 is dispensable for autotrophic growth of Arabidopsis thaliana. Journal of Experimental Botany 65 (9), 2405–2413.
→HopZ4 from Pseudomonas syringae, a member of the HopZ type III effector family from the YopJ superfamily, inhibits the proteasome.
Üstün, S.; König, P.; Gutmann, D.S.; Börnke, F. 2014. HopZ4 from Pseudomonas syringae, a member of the HopZ type III effector family from the YopJ superfamily, inhibits the proteasome. Molecular Plant-Microbe Interactions 27 (7) 611-623.
→The complex becomes more complex: protein-protein interactions of SnRK1 with DUF581 family proteins provide a framework for cell- and stimulus type-specific SnRK1 signaling in plants.
Nietzsche, M.; Schießl, I.; Börnke, F. 2014. The complex becomes more complex: protein-protein interactions of SnRK1 with DUF581 family proteins provide a framework for cell- and stimulus type-specific SnRK1 signaling in plants. Frontiers in Plant Science, doi: 10.3389/fpls.2014.00054
http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00054/abstract
Interactions of Xanthomonas type-III effector proteins with the plant ubiquitin and ubiquitin-like pathways.
Üstün, S.; Börnke, F. 2014. Interactions of Xanthomonas type-III effector proteins with the plant ubiquitin and ubiquitin-like pathways. Frontiers in Plant Science, doi: 10.3389/fpls.2014.00736.
http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00736/abstract
The Xanthomonas campestris Type III effector XopJ targets the host cell proteasome to suppress salicylic-acid mediated plant defence.
Üstün, S.; Bartetzko, V.; Börnke, F. 2013. The Xanthomonas campestris Type III effector XopJ targets the host cell proteasome to suppress salicylic-acid mediated plant defence. PLoS Pathogens 9(6): e1003427.
Funktionelle Charakterisierung von Typ-III Effektorproteinen aus Xanthomonas campestris pv. vesicatoria in Pflanzen.
van Thiel, D. 2016. Funktionelle Charakterisierung von Typ-III Effektorproteinen aus Xanthomonas campestris pv. vesicatoria in Pflanzen. TU Braunschweig, Fachrichtung Biologie, Master Thesis.
Identifizierung und Charakterisierung neuer Komponenten der SnRK1-Signaltransduktion in Arabidopsis thaliana.
Nietzsche, M. 2016. Identifizierung und Charakterisierung neuer Komponenten der SnRK1-Signaltransduktion in Arabidopsis thaliana. Universität Potsdam, Mathematisch-Naturwissenschaftliche Fakultät, 182 pp, PhD Thesis.
A remorin from Nicotiana benthamiana interacts with the Pseudomonas type-III effector protein HopZ1a and is phosphorylated by the immune-related kinase PBS1
Albers, P.; Üstün, S.; Witzel, K.; Kraner, M.; Börnke, F. 2018. A remorin from Nicotiana benthamiana interacts with the Pseudomonas type-III effector protein HopZ1a and is phosphorylated by the immune-related kinase PBS1. bioRxiv 409235.
DOI:
Identification and Characterization of Brassica oleracea Specifier Proteins in the Enzymatic Degradation of Glucosinolates
Risha, M.A.; Witzel, K.; Albers, P.; Schreiner, M.; Hanschen, F.S. 2017. Identification and Characterization of Brassica oleracea Specifier Proteins in the Enzymatic Degradation of Glucosinolates. 4th International Glucosinolate Conference, Book of Abstracts, 64.
The Pseudomonas springiae type III effector HOPZ1A targets a remorin possibly implicated in defense signaling.
Albers, Ph.; Üstün, S.; Börnke, F. 2015. The Pseudomonas springiae type III effector HOPZ1A targets a remorin possibly implicated in defense signaling. International Symposium „Communication in Plants and their Responses to the Environment“, Halle (Saale), Program and Abstratcs, 72.
MARD1/DUF19 is a possible mediator of crosstalk between energy and defense signaling in Arabidopsis.
Landgraf, R.; Niietzsche, M.; Börnke, F. 2015. MARD1/DUF19 is a possible mediator of crosstalk between energy and defense signaling in Arabidopsis. International Symposium „Communication in Plants and their Responses to the Environment“, Halle (Saale), Program and Abstratcs, 88.
The putative transcription factor STKR1: A novel component of SNRK1 mediated signaling?
Nietzsche, M.; Börnke, F. 2015. The putative transcription factor STKR1: A novel component of SNRK1 mediated signaling? International Symposium „Communication in Plants and their Responses to the Environment“, Halle (Saale), Program and Abstratcs, 92.
Wie Pflanzen krank werden.
Börnke, F. 2015. Wie Pflanzen krank werden. Jahresbericht 2013/2014 des Leibniz-Institutes für Gemüse- und Zierpflanzenbau Großbeeren/Erfurt, 39.
The putative transcription factor STKR1: A novel component of SNRK1 mediated signaling?
Nietzsche, M.; Korpys, A.; Börnke, F. 2015. The putative transcription factor STKR1: A novel component of SNRK1 mediated signaling? The 26th International Conference on Arabidopsis Research, Paris, Program and Abstratcs, 57.
The putative transcription factor STKR1: A novel component of SNRK1 mediated signaling?
Nietzsche, M.; Korpys, A.; Börnke, F. 2015. The putative transcription factor STKR1: A novel component of SNRK1 mediated signaling? Botanikertagung 2015, Weihenstephan, Conference Book, 122.
http://www.botanikertagung2015.de/
The plant proteasome is required for local and systemic defense responses and acts as a virulence target of bacterial type-III effector proteins.
Üstün, S.; Ntoutakis, V.; Börnke, F. 2015. The plant proteasome is required for local and systemic defense responses and acts as a virulence target of bacterial type-III effector proteins. Botanikertagung 2015, Weihenstephan, Conference Book, 85.
Aktuelles Projekt
Modulation des Trehalose-Stoffwechsels zur Erhöhung der Stresstolerance der Tomato (Acronym “opTOMize”)
Ziel des Vorhabens ist es durch ein erweitertes Verständis der molekularen Grundlagen der pflanz...
Weitere Projekte
Funktionelle Charakterisierung bakterieller Typ-III Effektoren in Pflanzen
Eine Reihe Gram-negativer pathogener Bakterien injiziert sogenannte Typ-III Effektorproteine in ihre eukaryontische Wirtszelle, um deren Abwehr zu supprimier...
Molekulare Anpassungen an Umwelteinflüsse
Die Aufrechterhaltung des physiologischen Gleichgewichtes unter fluktuierenden Umweltbedingungen ist für alle Organismen lebensnotwendig. In Eukaryoten wird...