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Specific poly phenolic compounds in cell culture of Vitis vinifera L. cv. Gamay Fréaux.

Cell cultures established from plants represent an attractive alternative to whole plants for effective production of bioactive secondary metabolites. Cell culture from Vitis vinifera L. cv. Gamay Fréaux accumulated high amounts of hydroxycinnamic acid derivatives and anthocyanins. Two new compounds were identified: 3-O-glucosylresveratrol – a stilbene derivative, abundant in cell suspension culture – and a hydroxyphenole, 4-(3,5-dihydroxyphenyl)-phenol, abundant in callus culture. The major anthocyanin monoglucosides present in cell suspension culture were cyanidin 3-O-glucoside and peonidin 3-O-glucoside and major cinnamoyl derivatives were cyanidin 3-O-p-coumarylglucoside and peonidin 3-O-p-coumarylglucoside. Three minor anthocyanin compounds were found in V. vinifera cell culture; delphinidin 3-O-glucoside, petunidin 3-O-glucoside, and delphinidin 3-O-p-coumarylglucoside. Anthocyanin levels of cell suspension cultures increased significantly – about eight-fold – after four-day cultivation in new medium. Salicylic acid at a concentration of 50 µM did not enhance anthocyanin accumulation in cell suspension culture, and similar levels of jasmonic acid reduced significantly the anthocyanin content.

Mewis, I.; Smetanska, I.M.; Müller, C.; Ulrichs CH. 2011. Specific poly-phenolic compounds in cell culture of Vitis vinifera L. cv. Gamay Fréaux. Applied Biochemistry and Biotechnology 164 (2), 148-161.