Induction of desiccation-tolerance in somatic embryos of Cyclamen persicum Mill..
As a first step in the development of synthetic seed of Cyclamen persicum Mill. various procedures for induction of desiccation-tolerance in somatic embryos of cyclamen have been tested. Most important proved to be the careful selection of mature embryos by morphological characteristics. Torpedo-shaped somatic embryos with a size between 700 and 1000 µm exhibited the highest germination rate after desiccation to only 0.2 g H20 g-1 DW. Addition of 75 g -1 polyethylene glycol (PEG 4000) and 10 mg-1abscisic acid to the maturation medium increased the germination rate of somatic embryos after desiccation to 28% compared to 4% in the control without these supplements.
Seyring, M.; Hohe, A. 2005. Induction of desiccation-tolerance in somatic embryos of Cyclamen persicum Mill.. Journal of Horticultural Science & Biotechnology 80 (1), 65-69.