Identification of heavy metal induced genes encoding glutathione S-transferases in the arbuscular mycorrhizal fungus Glomus intraradices.
Arbuscular mycorrhizal fungi are able to alleviate the stress for plants caused by heavy metal contamination of soil. To analyze the molecular response of arbuscular mycorrhizal fungi to these pollutants, a subtractive cDNA library was constructed using RNA from Glomus intraradices extraradical hyphae of a root organ culture treated with a mixture of Cd, Zn, and Cu. Screening by reverse Northern blot analysis indicated that, among 308 clones, 17% correspond to genes up-regulated by heavy metals. Sequence analysis of part of the clones resulted, amongst others, in the identification of six genes putatively coding for glutathione S-transferases belonging to two different classes of these enzymes. Expression analyses indicated that the genes are differentially expressed during fungal development and that their RNA accumulation dramatically increases in extraradical hyphae grown in a heavy metal-containing solution.
Waschke, A.; Sieh, D.; M. Tamasloukht, M.; Fischer, K.; Mann, P.; Franken. P. 2006. Identification of heavy metal induced genes encoding glutathione S-transferases in the arbuscular mycorrhizal fungus Glomus intraradices. Mycorrhiza 17 (1), 1-10.