Extraction extraradical arbuscular mycorrhizal mycelium from compartments filled with soil and glass beads.
This study presents a novel method for the extraction and quantification of extraradical mycelium (ERM) of arbuscular rnycolThizal ftlngi (AMP) from a Substrate that simulates soil better than previously used artificial growth media. Fungal compartments were constructed from small net pots with a latticed wall and filied with a mixture ofglass beads and 40 um wet sieved soil. The net pots were surrounded by a 30-~mm mesh membrane through which hyphae but not roots could grow. They were inserted into soil where a Glomus intraradices (BEG 110) colonized potato plant was growing. The ERM that had grown out from roots through the membrane was suecessfully collected and quantified after harvest by washing out the soil/glass bead mixture through a sieve with a mesh width of40 um. Concentrations of P, Zn, Cu and Mn in the AMF ERM were analysed.
Neumann, E.; George, E. 2005. Extraction extraradical arbuscular mycorrhizal mycelium from compartments filled with soil and glass beads. Mycorrhiza 15(7), 533-537.