Expression analysis of putative genes of pectin modifying enzymes in different callus lines of Cyclamen persicum and their correlation with the callus texture.
Gene expression of four different pectin modifying enzymes (a putative Pectin acetylesterase (PAE) homologue, a putative Pectin esterase (PE) homologue and two different putative Pectate lyase (PL) homologues) was examined in different callus cell lines of Cyclamen persicum as well as in the corresponding explants using quantitative PCR. Transcript abundances were analysed with special regard to potential correlations with callus texture and embryogenic competence. No correlation has been detected between gene expression of the putative pectin modifying enzymes and the embryogenic competence of the different cell lines. However, expression of the putative PAE homologue correlated with the callus texture: transcript abundances of the putative PAE homologue were higher the more friable the tissue was, in callus as well as in explant tissue. Histological analyses supported the conclusion that callus texture was influenced by the explant type.
Hoenemann, C.; Krueger, K.; Hohe, A. 2012. Expression analysis of putative genes of pectin modifying enzymes in different callus lines of Cyclamen persicum and their correlation with the callus texture. Scientia Horticulturae 140 (1), 52-59.