Cloning and analysis of psam2, a gene from Pisum sativum L. regulated in symbiotic arbuscular mycorrhiza and pathogenic root-fungus interactions.
A differential RNA display fragment was used to isolate a full size clone from a cDNA library prepared from Pisum sativum L. root RNA. The corresponding gene, psam2, belongs to a gene family and its RNA accumulation was repressed in roots after inoculation with the arbuscular mycorrhizal fungus Glomus mosseae. No significant changes in transcript levels could be observed in the nodule symbiosis formed with Rhizobium. RNA accumulation was, however, enhanced after infection with two fungal root pathogens, Chalara elegans and Aphanomyces euteiches. Phosphate or nitrate fertilization had no influence on psam2 RNA expression. Sequencing revealed that the putative gene product is similar to animal secretory carrier membrane proteins. Its possible involvement in changes in root exuded compounds after mycorrhizal formation or in secretion processes in phytopathogenic interactions is discussed. (C) 1998 Academic Press.
Krajinski, F.; Martin-Laurent, F.; Gianinazzi, S.; Gianinazzi-Pearson, V.; Franken, P. 1998. Cloning and analysis of psam2, a gene from Pisum sativum L. regulated in symbiotic arbuscular mycorrhiza and pathogenic root-fungus interactions. Physiological and Molecular Plant Pathology 52 (5), 297- 307.