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Mass spectrometry-based exploration of plant-associated microbial communities

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Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) is a unique tool for fast and high resolving characterization and identification of cultivable microorganisms. In this MS-based biotyping technique, the molecular mass of most abundant proteins in a crude extract is measured and the spectra information is used as a fingerprint for a particular organism.

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The method is applied for the rapid grouping of bacterial and fungal isolates, i.e., dereplication, in large-scale screening campaigns of microorganisms isolated from plants grown under diverse environmental conditions, without loss of biodiversity. Isolates collected from rhizospheres and plants are tested in inoculation experiments for their potential use to sustainably increase crop production.

Other Projects

Spatial mapping of root metabolites

Plant roots secrete a wide range of compounds that govern the interaction with other organisms in the rhizosphere. In this project, the spatial dis...

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Elucidating plant-pathogen interactions at the protein level

Soil-borne pathogenic fungi cause severe losses in crop yield and quality. In Principle, they colonize the plant root...

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DiControl

Implications of soil management practices and application of biocontrol strains on soil disease suppressiveness for i...

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