Moringa oleifera establishment and multiplication of different ecotypes in vitro.
To obtain healthy plant material from Moringa oleifera regardless of the season, weather, and degree of infestation, a procedure to establish an in vitro culture of M. oleifera from nodes using 0.2% mercury chloride was developed. It was not possible to create an in vitro culture of Moringa from seeds. Nodes were cultivated on MS medium with different concentrations of benzylaminopurine (BAP) and agar contents to find the best conditions for rapid growth and optimum multiplication. The highest multiplication ability of the different plant parts, especially the base parts of Moringa in vitro plants, was observed after three weeks of cultivation on MS medium with 0.5 mg l-1 BAP. Callus formation increases with increased BAP concentration (0, 0.5, 0.75, 1 mg l-1 BAP). Furthermore, the use of two phytohormones – indole-3-acetic acid and thidiazuron – led to very strong callus formation of adaxial and abaxial leaves on MS medium. This formation was only observed for material that was light induced for 24 hours prior to cultivation under dark conditions. Analysis of the glucosinolate content of Moringa leaves revealed a different glucosinolate profile of plants cultivated in vitro and in soil beds in the greenhouse. Whereas in greenhouse leaves rhamnopyranosyloxy-benzyl-glucosinolates were abundant, the precursor benzyl-glucosinolate was found in vitro cultures.
Förster, F.; Mewis, I.; Ulrichs, C. 2013. Moringa oleifera establishment and multiplication of different ecotypes in vitro. Gesunde Pflanzen 65 (1), 21-31.