Dr. rer. nat. Katja Witzel
Dr. rer. nat.
Katja Witzel
Großbeeren
033701 - 78 220
witzel@igzev.de
Scientist
Ausbildung
Berufliche Tätigkeiten
Sonstige Tätigkeiten
Elucidating plant-pathogen interactions at the protein level
Verticillium species are destructive vascular wilt fungi with worldwide distribution, causing severe losses in crop yield and Quality....
Identification of traits conferring abiotic stress tolerance in crops and model plants
Salinity is one of the most severe abiotic factors threatening agriculture worldwide....
Plant Proteomics Facility |
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Research profile |
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The research focuses on characterizing regulatory networks in plant biotic and abiotic stress defense at the proteome level. In a range of projects, methods are developed for targeted and non-targeted proteomic analysis of plant tissues (leaves, roots, grains) and subcellular structures (microsomal membranes, plasma membrane, apoplastic fluid). Together with cooperation partners we establish workflows for mapping post-translational modifications of proteins and peptides. A MALDI-TOF-based method for the identification of microorganisms from plant and environmental samples adds to the portfolio. |
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Professional appointments |
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| 2011 – present | Leibniz-Institute of Vegetables and Ornamental Crops, Department of Plant Nutrition, Senior Scientist (since 2014 awarded as permanent Position) |
| 2010-2011 | Christian-Albrechts-University Kiel, Institute of Plant Nutrition and Soil Science, Postdoctoral Research Fellow |
| 2004-2009 | Leibniz-Institute of Plant Genetics and Crop Plant Research, Research Group Applied Biochemistry, PhD student and Postdoctoral Research Fellow |
| 2003-2004 | Leibniz-Institute of Plant Genetics and Crop Plant Research, Research Group Molecular Plant Physiology, Junior Scientist |
| 2003 | Friedrich-Schiller-University Jena, Department of Biology and Pharmacy, Graduate Research Assistant |
Education |
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| 2008 | PhD, Biology / Biochemistry
Martin-Luther-University of Halle, Germany, Natural Sciences Faculty Dissertation: “A proteomic approach to investigate agronomic traits in barley using the genetic variation of mapping populations”, summa cum laude |
| 2003 | Diploma, Biology
Friedrich-Schiller-University of Jena, Germany, Biology and Pharmacy Faculty Thesis: “Comparative analysis of gene expression of prenyltransferases in Norway spruce (Picea abies)”, Max-Planck-Institute of Chemical Ecology, Department of Biochemistry, Jena |
Differences in the enzymatic hydrolysis of glucosinolates increase the defense metabolite diversity in 19 Arabidopsis thaliana accessions
Hanschen, F.S.; Pfitzmann, M.; Witzel, K.; Stützel, H.; Schreiner, M.; Zrenner, R. 2018. Differences in the enzymatic hydrolysis of glucosinolates increase the defense metabolite diversity in 19 Arabidopsis thaliana accessions. Plant Physiology and Biochemistry 124, 126-135.
DOI: doi.org/10.1016/j.plaphy.2018.01.009
→Plasma membrane proteome analysis identifies a role of barley membrane steroid binding protein in root architecture response to salinity
Witzel, K.;, Matros, A.; Møller, A.L.B.; Ramireddy, E.; Finnie, C.; Peukert, M.; Rutten, T.; Herzog, A.; Kunze, G.; Melzer, M.; Kaspar-Schoenefeld, S.; Schmülling, T.; Svensson, B.; Mock, H-P 2018. Plasma membrane proteome analysis identifies a role of barley membrane steroid binding protein in root architecture response to salinity. Plant, Cell & Environment
DOI: https://doi.org/10.1111/pce.13154
→A proteomic approach suggests unbalanced proteasome functioning induced by the growth-promoting bacterium Kosakonia radicincitans in Arabidopsis.
Witzel, K.; Üstün, S.; Schreiner, M.; Grosch, R.; Börnke, F.; Ruppel, S. 2017. A proteomic approach suggests unbalanced proteasome functioning induced by the growth-promoting bacterium Kosakonia radicincitans in Arabidopsis. Frontiers in Plant Science 2017 8:661.
→Arabidopsis thaliana root and root exudate metabolism is altered by the growth-promoting bacterium Kosakonia radicincitans DSM 16656T
Witzel, K.; Strehmel, N.; Baldermann, S.; Neugart, S.; Becker, Y.; Becker, M.; Berger, B.; Scheel, D.; Grosch, R.; Schreiner, M.; Ruppel, S. 2017. Arabidopsis thaliana root and root exudate metabolism is altered by the growth-promoting bacterium Kosakonia radicincitans DSM 16656T. Plant and Soil 419, 557-573.
→Metabolic profiling of glucosinolates and their hydrolysis products in a germplasm collection of Brassica rapa turnips.
Klopsch, R., Witzel, K., Börner, A., Schreiner, M. & Hanschen, F. S. 2017. Metabolic profiling of glucosinolates and their hydrolysis products in a germplasm collection of Brassica rapa turnips. Food Research International 100, 392-403.
→Natural diversity of hydroxycinnamic acid derivatives, flavonoid glycosides, carotenoids and chlorophylls in leaves of six different amaranth species
Schröter, D.; Baldermann, S.; Schreiner, M.; Witzel, K.; Maul, R.; Rohn, S.; Neugart, S. 2017. Natural diversity of hydroxycinnamic acid derivatives, flavonoid glycosides, carotenoids and chlorophylls in leaves of six different amaranth species. Food Chemistry
DOI: https://doi.org/10.1016/j.foodchem.2017.11.043
→Site-directed mutagenesis to deactivate two nitrogenase isozymes of Kosakonia radicincitans DSM16656T
Ekandjo, L.K.; Ruppel, S.; Remus, R.; Witzel, K.; Patz, S.; Becker, Y. 2017. Site-directed mutagenesis to deactivate two nitrogenase isozymes of Kosakonia radicincitans DSM16656T. Canadian Journal of Microbiology.
DOI: https://doi.org/10.1139/cjm-2017-0532
→Systemic induction of salicylic acid-related plant defences in potato in response to Rhizoctonia solani AG3PT.
Genzel, F.; Franken, P.; Witzel, K.; Grosch, R. 2018. Systemic induction of salicylic acid-related plant defences in potato in response to <i>Rhizoctonia solani</i> AG3PT. Plant Pathology 67, 337 – 348.
DOI: 10.1111/ppa.12746
→Temporal impact of the vascular wilt pathogen Verticillium dahlia on tomato root proteome.
Witzel, K.; Buhtz, A.; Grosch, R. 2017. Temporal impact of the vascular wilt pathogen Verticillium dahlia on tomato root proteome. Journal of Proteomics, 169, 215-224 .
https://www.ncbi.nlm.nih.gov/pubmed/28428141
→Interactive effects of genotype and N/S-supply on glucosinolates and glucosinolate breakdown products in Chinese cabbage (Brassica rapa L. ssp. pekinensis).
Geilfus, C.M.; Hasler, K.; Witzel, K.; Gerendás, J.; Mühling, K.H. 2016. Interactive effects of genotype and N/S-supply on glucosinolates and glucosinolate breakdown products in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Journal of Applied Botany and Food Quality 89, 279 – 286.
→Rhizosecretion of stele-synthesized glucosinolates and their catabolites requires GTR-mediated import in Arabidopsis.
Xu, D.; Hanschen, F.S.; Witzel, K.; Nintemann, S.J.; Nour-Eldin, H.H.; Schreiner, M.; Halkier, B.A. 2016. Rhizosecretion of stele-synthesized glucosinolates and their catabolites requires GTR-mediated import in Arabidopsis. Journal of Experimental Botany 2016 erw 355, 1-10.
http://jxb.oxfordjournals.org/content/early/2016/10/03/jxb.erw355.abstract
doi: 10.1093/jxb/erw355
→Verticillium longisporum infection induces organ-specific glucosinolate degradation in Arabidopsis thaliana.
Witzel, K.; Hanschen, F.S.; Klopsch, R.; Ruppel, S.; Schreiner, M.; Grosch, R. 2015. Verticillium longisporum infection induces organ-specific glucosinolate degradation in Arabidopsis thaliana. Frontiers in Plant Science 6:508.
http://journal.frontiersin.org/article/10.3389/fpls.2015.00508/abstract
→Perturbations in the primary metabolism of tomato and i>Arabidopsis thaliana plants infected with the soil-borne fungus Verticillium dahliae.
Buhtz, A.; Witzel, K.; Strehmel, N.; Ziegle, J.; Abel, S.; Grosch, R. 2015. Perturbations in the primary metabolism of tomato and Arabidopsis thaliana plants infected with the soil-borne fungus Verticillium dahliae. PLoS ONE 10(9):e0138242.
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138242
→Recent progress in the use of ‘omics technologies in brassicaceous vegetables.
Witzel, K.; Neugart, S.; Ruppel, S.; Schreiner, M.; Wiesner, M.; Baldermann, S. 2015. Recent progress in the use of ‘omics technologies in brassicaceous vegetables. Frontiers in Plant Science 6:244.
Recent progress in the use of ‘omics technologies in brassicaceous vegetables
Witzel, K.; Neugart, S.; Ruppel, S.; Schreiner, M.; Wiesner, M.; Baldermann, S. 2015. Recent progress in the use of ‘omics technologies in brassicaceous vegetables. Frontiers in Plant Science 6:244.
DOI: 10.3389/fpls.2015.00244
→Salinity stress in roots of contrasting barley genotypes reveals time-distinct and genotype-specific patterns for defined proteins.
Witzel, K.; Matros, A.; Strickert, M.; Kaspar, S.; Peukert, M.; Mühlin, K.H.; Börner, A.; Mock, H.-P. 2014. Salinity stress in roots of contrasting barley genotypes reveals time-distinct and genotype-specific patterns for defined proteins. Molecular Plant 7, 336-355.
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Elucidation of salt stress defense and tolerance mechanisms of crop plants using proteomics – Current achievements and perspectives.
Barkla, B. J.; Castellanos-Cervantes, T.; Diaz de León, J. L.; Matros, A.; Mock, H. P.; Perez-Alfocea, F; Salekdeh, G. H.; Witzel, K.; Zörb, C. 2013. Elucidation of salt stress defense and tolerance mechanisms of crop plants using proteomics – Current achievements and perspectives. Proteomics 13 (12-13), 1885-1900.
→Properties of the halophyte microbiome and their implications for plant salt tolerance.
Ruppel, S.; Franken, P.; Witzel, K. 2013. Properties of the halophyte microbiome and their implications for plant salt tolerance. Functional Plant Biology 40, 940-951.
http://www.publish.csiro.au/paper/FP12355.htm
Verticillium suppression is associated with the glucosinolate composition of Arabidopsis thaliana leaves.
Witzel, K.; Hanschen, F.S.; Schreiner, M.; Krumbein, A.; Ruppel, S.; Grosch, R. 2013. Verticillium suppression is associated with the glucosinolate composition of Arabidopsis thaliana leaves. PLOS ONE 2013, PLoS ONE 8(9): e71877. doi:10.1371/journal.pone.0071877 0071877
Genome sequence of Enterobacter radicincitans DSM16656T, a plant Ggrowth-promoting endophyte.
Witzel, K.; Gwinn-Giglio, M.; Nadendla, S.; Shefchek, K.; Ruppel, S. 2012. Genome sequence of Enterobacter radicincitans DSM16656T, a plant Growth-promoting endophyte. Journal of Bacteriology 194 (19), 5469.
→Determination of oxidative stress in wheat leaves as influenced by boron toxicity and NaCl stress: Luminescence Assays.
Masood, S.; Saleh, L.; Witzel, K.; Plieth, C.; Mühling, K.H. 2012. Determination of oxidative stress in wheat leaves as influenced by boron toxicity and NaCl stress: Luminescence Assays. Plant Physiology and Biochemistry 56, 56-61.
→Growth-related changes in subcellular ion patterns in maize leaves (Zea mays L.) under salt stress.
Shahzad, M.; Witzel, K.; Zörb, C.; Mühling, KH. 2012. Growth-related changes in subcellular ion patterns in maize leaves (Zea mays L.) under salt stress. Journal of Agronomy and Crop Science 198 (1), 46-56.
DOI: 10.1111/j.1439-037X.2011.00487.x
→Interactive effects of high boron and NaCl stresses on subcellular localization of chloride and boron in wheat leaves.
Masood, S.; Wimmer, M. A.; Witzel, K.; Zörb, C.; Mühling, K. H. 2012. Interactive effects of high boron and NaCl stresses on subcellular localization of chloride and boron in wheat leaves. Journal of Agronomy and Crop Science 198 (3), 227-235.
→Comparative evaluation of extraction methods for apoplastic proteins from maize leaves.
Witzel, K.; Shahzad, M.; Matros, A.; Mock, H-P.; Mühling KH. 2011. Comparative evaluation of extraction methods for apoplastic proteins from maize leaves. Plant Methods 7 (48), doi:10.1186/1746-4811-7-48 →
Proteomic approaches to understand plant growth promotion caused by the endophyte Enterobacter radicincitans sp. nov. DSM 16656.
Witzel, K.; Ruppel, S. 2013. Proteomic approaches to understand plant growth promotion caused by the endophyte Enterobacter radicincitans sp. nov. DSM 16656. European Proteomics Association 2013 Scientific Meeting, 14.-17.10.2013, St. Malo, France, Abstractbook, S. 131-132.
Genomic and molecular background of plant growth promotion in the endophyte Enterobacter radicincitans sp. nov. DSM 16656.
Witzel, K.; Ruppel, S. 2013. Genomic and molecular background of plant growth promotion in the endophyte Enterobacter radicincitans sp. nov. DSM 16656. In: Carolin Schneider, Carlo Leifert, and Falko Feldmann (eds.), Endophytes for plant protection: the state of the art. DLG-Verlag, Berlin, 159.
Glucosinolate in der Modellpflanzen Arabidopsis thaliana – enzymatische Bioaktivierung.
Hanschen, F.S.; Pfitzmann, M.; Zrenner, R.; Witzel, K.; Stützel, H.; Rohn, S.; Schreiner, M. 2013. Glucosinolate in der Modellpflanzen Arabidopsis thaliana – enzymatische Bioaktivierung. 42. Deutscher Lebensmittelchemikertag 2013 – Lebensmittelchemische Gesellschaft – Fachgruppe in der GDCh 16.-18.09.2013, Braunschweig, Kurzreferate, S. 136.
ISBN 978-3-936028-80-5.
Mutualistische Interaktion zwischen Bakterien und Pflanzen.
Brock, A.; Berger, B.; Witzel, K.; Ruppel, S. 2013. Mutualistische Interaktion zwischen Bakterien und Pflanzen. Jahresbericht 2011/2012 des Leibniz-Institutes für Gemüse- und Zierpflanzenbau Großbeeren/Erfurt, S. 50-51.
Genetic variability of the Verticillium syndrome in an Arabidopsis mapping population
Witzel, K.; Schreiner, M.; Ruppel, S.; Grosch, R. 2012. Genetic variability of the Verticillium syndrome in an Arabidopsis mapping population. 28th New Phytologist Symposium: Functions and Ecology of the Plant Microbiome, 18.05.-21.05.2012, Rhodes, Greece, Abstractbook, S. 76.
Enterobacter radicincitans sp.nov. DSM 16656: a novel and efficient PGPB strain
Witzel, K.; Brock, A.; Berger, B.; Ruppel, S. 2012. Enterobacter radicincitans sp.nov. DSM 16656: a novel and efficient PGPB strain. 10th European Nitrogen Fixation Conference, Munich, Germany, 02.09.-05.09.2012, Abstractband.
Localisation and quantification of the PGPB Enterobacter radicincitans sp. nov. DSM 16656 in crop and model plants.
Witzel, K.; Berger, B.; Ruppel, S. 2012. Localisation and quantification of the PGPB Enterobacter radicincitans sp. nov. DSM 16656 in crop and model plants. COST Meeting Endophytes 14.11.-16.11.2012., Trento San Michele, Italy, Book of abstracts, 59.
Microbial plant interactions in saline habitats.
Ruppel, S.; Franken, P.; Witzel, K. 2012. Microbial plant interactions in saline habitats. COST WG2 Meeting: Putting Halophytes to Work – Genetics, Biochemistrys, Physiology 29.-30.08.2012, Hannover. Lectures and Posters, Abstract 14.
Bestimmung von Glucosinolaten und Isothiocyanaten in Brassica pekinensis nach steigendem S-Angebot – Einfluss auf die Nrf2-Transaktivierung in HT-29 Zellen.
Heyer, C.; Reichelt, M.; Witzel, K.; Wagner, A.; Rimbach, G.; Mühling, K.H. 2011. Bestimmung von Glucosinolaten und Isothiocyanaten in Brassica pekinensis nach steigendem S-Angebot - Einfluss auf die Nrf2-Transaktivierung in HT-29 Zellen. Gemeinsame Tagung der Deutschen Gesellschaft für Pflanzenzernährung e. V. und der Gesellschaft für Pflanzenbauwissenschaften e. V., Stickstoff in Pflanze, Boden und Umwelt, Kiel, Deutschland, 27.09.-29.09.2011, Abstract, 255.
Einfluss einer S-Düngung auf Ertrags- und Qualitätsparameter von zwei Weizensorten im Feldversuch unter besonderer Berücksichtigung von S-Metaboliten.
Betzinger, R.; Witzel, K.; Feger, G.; Seling, S.; Mühling, K.H. 2011. Einfluss einer S-Düngung auf Ertrags- und Qualitätsparameter von zwei Weizensorten im Feldversuch unter besonderer Berücksichtigung von S-Metaboliten. Gemeinsame Tagung der Deutschen Gesellschaft für Pflanzenzernährung e. V. und der
Gesellschaft für Pflanzenbauwissenschaften e. V., Stickstoff in Pflanze, Boden und Umwelt, Kiel, Deutschland, 27.09.-29.09.2011, Abstract, 251.
Interaction of salinity and boron toxicity on ion relations, antioxidants and protein pattern of wheat leaves.
Masood, S.; Wimmer, M.; Witzel, K.; Zörb, C.; Plieth, C.; Mühling, K.H. 2011. Interaction of salinity and boron toxicity on ion relations, antioxidants and protein pattern of wheat leaves. Gemeinsame Tagung der Deutschen Gesellschaft für Pflanzenzernährung e. V. und der Gesellschaft für Pflanzenbauwissenschaften e. V., Stickstoff in Pflanze, Boden und Umwelt, Kiel, Deutschland, 27.09.-29.09.2011, Abstract, 187.
Subcellular Proteomic Approaches to Evaluate Agronomic Traits in Crops.
Witzel, K.; Matros, A.; Finnie, C.; Mühling, K.H.; Mock, H.-P. 2011. Subcellular Proteomic Approaches to Evaluate Agronomic Traits in Crops. Botanikertagung 2011, Berlin, 19.09.-23.09.2011, Abstract, 199.
Mini-scale isolation and preparation of plasma membrane proteins from potato roots for LC/MS analysis
Jozefowicz, A.M.; Matros, A.; Witzel, K.; Mock, H-P 2018. Mini-scale isolation and preparation of plasma membrane proteins from potato roots for LC/MS analysis. In: Mock, H-P; Matros, A.; Witzel, K. (eds) Plant Membrane Proteomics: Methods and Protocols. Humana Press New York, NY, 195-204.
DOI: 10.1007/978-1-4939-7411-5_13
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Genotypic variation of glucosinolates and their hydrolysis products in a germplasm collection of Brassica rapa turnips
Klopsch, R.; Witzel, K.; Börner, A.; Schreiner, M.; Hanschen, F.S. 2017. Genotypic variation of glucosinolates and their hydrolysis products in a germplasm collection of Brassica rapa turnips. 4th International Glucosinolate Conference, Berlin, Germany, 17 – 20 September. Book of Abstracts, 84.
Identification and Characterization of Brassica oleracea Specifier Proteins in the Enzymatic Degradation of Glucosinolates
Risha, M.A.; Witzel, K.; Albers, P.; Schreiner, M.; Hanschen, F.S. 2017. Identification and Characterization of Brassica oleracea Specifier Proteins in the Enzymatic Degradation of Glucosinolates. 4th International Glucosinolate Conference, Book of Abstracts, 64.
A proteomic view of the cereal and vegetable crop response to salinity stress.
Witzel, K.; H.-P. Mock 2016. A proteomic view of the cereal and vegetable crop response to salinity stress. In: Salekdeh, G.H. (Ed.) Agricultural Proteomics Volume 2: Environmental Stresses, 53-69.
Differential expressions of anfH and nifH of Kosakonia radicincitans DSM16656T in nitrogen enriched media.
Ekandjo, L.; Becker, Y., Witzel, S.; Patz, S.; Remus, R.; Ruppel, S. 2016. Differential expressions of anfH and nifH of Kosakonia radicincitans DSM16656T in nitrogen enriched media. Black forrest summer school on NGS data for phylogenetics, Book of Abstracts, 17.
The role of anfH and nifH in biological nitrogen fixation of the endophytic plant growth promoting bacterium Kosakonia radicincitans.
Ekandjo, L.; Becker, Y., Witzel, S.; Patz, S.; Remus, R.; Ruppel, S. 2016. The role of anfH and nifH in biological nitrogen fixation of the endophytic plant growth promoting bacterium Kosakonia radicincitans. Euro plant biology congress, Book of Abstracts, 52.
The role of anfH and nifH on biological nitrogen fixation in the plant growth promoting bacterium Kosakonia radicincitans.
Ekandjo, L.; Becker, Y., Witzel, S.; Patz, S.; Remus, R.; Ruppel, S. 2016. The role of anfH and nifH on biological nitrogen fixation in the plant growth promoting bacterium Kosakonia radicincitans. 12 th European nitrogen cconference, Book of Abstracts, 27.
Einfluss von verschiedenen UV-Lichtspektren auf die Ausbildung von Sekundärmetaboliten in unterschiedlichen Entwicklungsstadien von Pak Choi.
Heinze, M.; Schreiner, M.; Baldermann, S.; Hanschen, F.S.; Ruppel, S.; Wiesner, M.; Witzel, K.;Mengdehl, M.; Garbe, L.A.; Neugart, S. 2015. Einfluss von verschiedenen UV-Lichtspektren auf die Ausbildung von Sekundärmetaboliten in unterschiedlichen Entwicklungsstadien von Pak Choi. 44. Deutscher Lebensmittelchemikertag, Karlsruhe, Book of Abstracts, 83. ISBN: 978-3-936028-93-5.
Effect of glucosinolate on Verticillium spread in Arabidopsis.
Witzel, K.; Hanschen, F.S.; Klopsch, R.; Ruppel , S.; Schreiner, M. Grosch, R. 2014. Effect of glucosinolate on Verticillium spread in Arabidopsis. Proceedings of the 3rd International Glucosinolate Conference 2014 “Glucosinolates and Beyond” 12.-15.10.2014, p.120.
ISBN: 978-94-6257-382-6.
Enzymatische Bioaktivierung der Glucosinolate in Brassica-Gemüse.
Hanschen, F.S.; Witzel, K.; Rohn, S.; Schreiner, M. 2014. Enzymatische Bioaktivierung der Glucosinolate in Brassica-Gemüse. 43. Deutscher Lebensmittelchemikertag, Gießen (Germany), 22.-24. September 2014, Tagungsband Kurzreferate ,177.
ISBN 978-3-936028-87-4
Transport processes in exudation of glucosinolates from Arabidopsis root to the rhizosphere.
Xu, D.; Hanschen, F.S.; Witzel, K.; Jørgensen, M.E.; Nour-Eldin, H.H.; Schreiner, M.; Halkier, B.A. 2014. Transport processes in exudation of glucosinolates from Arabidopsis root to the rhizosphere. Proceedings of the 3rd International Glucosinolate Conference 2014 “Glucosinolates and Beyond” 12.-15.10.2014, p. 121.
ISBN: 978-94-6257-382-6.
Plant Membrane Proteomics: Methods and Protocols
Mock, H-P; Matros, A.; Witzel, K. (eds) Plant Membrane Proteomics: Methods and Protocols. Humana Press New York, NY, 280 pp.